Protein_Domain
GSG

Part:BBa_K3183014:Design

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-16)


Gly-Ser-Gly Protein Domain Linker for E. coli

The linker is typically assembled onto larger parts during PCR, by including its sequence as an overhang to the appropriate primer. The PCR product can then be further assembled into more complex constructs by Gibson Assembly, such that the individual domains are spaced by a hydrophilic linked which allows them to fold independently.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Compatible to RF10, 12, 21, 23, 25, and 1000


Source

Oxford iGEM Team 2019 - de novo design

References